Bacillus subtilis "rec assay" test with isogenic strains.
نویسنده
چکیده
The Bacillus subtilis "rec assay" test, widely used for the detection of DNA-damaging agents, was studied in detail, using an isogenic set of strains carrying different mutations in repair or recombination functions or both. recE4 and rec-45 mutations turned out to confer on the cells the highest sensitivity to known mutagens. The recE4 strain and its isogenic rec+ strain have been tested and validated by several rec assay procedures for preferential killing by known DNA-damaging agents. The use of purified spores of the tester strains offers advantages for standardization of the assay.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 43 1 شماره
صفحات -
تاریخ انتشار 1982